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Screening error and need for quality assurance

Quality assurance is essential to minimise the risk of errors of cervical cytology

There are 3 main types of errors:

  1. Errors in smear taking.
  2. Errors in laboratory processing.
  3. Errors in interpretation.

Up to 10% of smears from women with pre-invasive or early invasive cancer of the cervix may not contain abnormal cells. There are three possible explanations for this:

  1. Errors of smear taking:
    1. The area of the transformation zone was not adequately sampled
    2. The abnormal cells were not transferred from spatula to slide
    3. The CIN lesion may be small and/or high in the endocervical canal beyond the reach of the spatula
  2. Errors of fixation and processing:
    1. Cell morphology not clearly displayed
    2. Nuclear/ cytoplasmic ratios distorted
    3. Nuclear outline and chromatin structure poorly displayed
    4. Pale nuclear staining and  loss of nuclear/ cytoplasmic contrast
    5. Clerical error in mislabeling of slide
  3. Errors in the interpretation of the smear:
    1. Loss of concentration when screening
    2. Habituation- A subconscious process by which the brain ignores a repeated signal
    3. Distraction from screening (a telephone call)
    4. Failure to screen the whole slide
    5. Fatigue (screening too many slides at a sitting)
    6. Unsatisfactory microscope optics or laboratory ergonomics
    7. Clerical error in recording results

Pitfalls of Diagnosis

False negative reports are issued when the cytologist fails to detect abnormal cells which are present in the smear .There have been several studies to determine the characteristics of false negative smears.  These have shown that  the risk of a false negative smear report being issued is very high if the smear contain relatively few ( < 50 ) abnormal cells.  Unfortunately studies have also shown that  the majority of  false negative smear reports are issued  in cases where the   smears contain  a very large number of abnormal cells indeed.  In fact , in retrospective studies of women who have presented with invasive cancer it has been found that  up to three or more consecutive false negative reports have been issued over a period of several years before  invasive cancer was diagnosed . False negative reports are particularly harmful  because they result in failure to diagnose or treat the patient’s cancer.

False Positive reports are issued when the negative smears are reported to contain abnormal cells . In these cases the patient may undergo unnecessary surgical procedure  and may be alarmed unnecessarily.
A number of conditions have been recognised which may give rise to  problems of interpretation  leading   to  a false positive diagnosis. These so called pitfalls in diagnosis are listed and several are illustrated below

Pitfalls in diagnosis

  1. Small cell dyskariosis
  2. Follicular lymphocytic  cervicitis
  3. CIN3 minibiopsies  ( microbiopsies ) or CIN involving crypts
  4. Discrete small keratinised cells
  5. Endometriosis , tubo endometrioid  metaplasia
  6. Lower segment endometrium
  7. Immature metaplastic cells
  8. Histiocytes
  9. radiation changes
Radiation changes : the anisonucleosis in this cluster of  
squamous  cells is marked but the nuclear structure is bland and the cytoplasm abundant. It is important to take note of the   clinical history when interpreting a smear.
Immature metaplastic cells: The slight anisonucleosis and hyperchromasia in this cluster of cells led to a diagnosis of ASCUS . Colposcopy and subsequent cytology were negative .
Immature metaplastic cells: The slight anisonucleosis and hyperchromasia in this cluster of cells led to a diagnosis of ASCUS . Colposcopy and subsequent cytology were negative .
These epithelial cells were present in a follow up smear from a woman who had LLETZ for CIN3.They were reported as suspicious of recurrence/residual CIN but colposcopy and follow up cytology was negative .They are probably immature metaplastic cells.

A dense cluster of poorly differentiated malignant squamous cells from a case of invasive squamous carcinoma . High power microscopy of the edge of the cluster reveals the crowded overlapping hyperchromatic nuclei which vary in shape and size and have abnormal chromatin content.

A dense cluster of poorly differentiated malignant squamous cells from a case of invasive squamous carcinoma . High power microscopy of the edge of the cluster reveals the crowded overlapping hyperchromatic nuclei which vary in shape and size and have abnormal chromatin content.
This smear was from a case of invasive squamous carcinoma. Note the numerous discrete small keratinised cells Note also the malignant diathesis. Elsewhere in the smear there were severely dyskariotic cells.

 

NOTE: Up to 45% of smears from women with advanced invasive cancer may not contain abnormal cells . This is because the lesions are often  necrotic and ulcerating and the smear is comprised  entirely of  blood , polymorphs and necrotic debris (the so called malignant diathesis) Cytotechnologists can minimise this problem by systematically evaluating the smears for their adequacy and advising smear takers of the quality of their smears.