Rapid on-site evaluation single slide assessment Archives

Training plans and competency assessment

An effective training plan is vital in ROSE. The individual needs to obtain the skills to feel confident in their decision making and remain calm in a pressured environment. Selection of staff with the right aptitude is crucial. A comprehensive training programme should include the following: Laboratory training: Multi header microscope sessions to include: Overview […]

ROSE SSA – Head and Neck / palpable masses

Images first published: Glinski et al. Single slide assessment: A highly effective cytological rapid on?site evaluation technique for endobronchial and endoscopic ultrasound?guided fine needle aspiration. Cytopathology 30, 164–172 (2019). Diagnostic pitfalls and considerations

ROSE SSA – EUS Pancreas

Images first published: Glinski et al. Single slide assessment: A highly effective cytological rapid on?site evaluation technique for endobronchial and endoscopic ultrasound?guided fine needle aspiration. Cytopathology 30, 164–172 (2019). ROSE SSA is utilised for solid lesions in the pancreas. If mucin is present in the adequacy assessed pancreatic preparation or there is a clinical suspicion of a cystic lesion, […]

ROSE SSA Lung—EBUS/EUS mediastinal

Images first published: Glinski et al. Single slide assessment: A highly effective cytological rapid on?site evaluation technique for endobronchial and endoscopic ultrasound?guided fine needle aspiration. Cytopathology 30, 164–172 (2019). There are many personalised therapies available for advanced lung carcinomas. ROSE SAA is vital in this area to establish that the correct diagnostic cell yield is obtained. Tumour type Approved […]

Equipment required and conduct in the clinical setting

Consumables and equipment Consumables Solutions Equipment Patient request form CytoLyt solution Hair dryer Personal protective equipment Rapid MGG stains Microscope 1 ml Eppendorf Pipette tips Sterile Saline Pen and pencil 5 ml syringes (saline flush) Deionised water Drip and rinse tray Glass microscope slides Vial rack (prevents vial being knocked over) Slide boxes and […]

ROSE SSA Technique

General suction advice: (only applicable to EBUS and EUS) Lymph nodes: suction is not used on lymph nodes on the first pass to prevent excess blood contaminant in the sample. Excess blood results in dilution of the sample and clotting, making ROSE slide preparation difficult and increasing the likelihood of diagnostic cellular material being encapsulated […]

Introduction

The purpose of rapid on-site evaluation single slide assessment (ROSE SSA) is to determine the diagnostic adequacy and viable yield of cytology samples in real time, allowing correct sample triage and optimal preservation of the material, ensuring that a complete diagnosis and patient eligibility for personalised targeted therapies can be established in one minimally invasive […]